Expression of cytochrome P4502E1 gene in hepatocellular carcinoma
作者： Xiao-Bo Man , Liang Tang , Xiu-Hua Qiu , Li-Qun Yang , Hui-Fang Cao
关键词:
摘要: AIM: To investigate cytpchrome P4502E1 (CYP2E1) gene expression in occurrence and progression of hepatocellular carcinoma (HCC). METHODS: The human liver arrayed library was spotted onto the nylon membranes to make cDNA array. Hybridization array performed with labeled probes synthesized from RNA isolated HCC adjacent tissues. Sprague-Dawley rats were administrated diethylnitrosamine (DENA) induce HCC. CYP2E1 detected by method RT-PCR Northern blot analysis. RESULTS: found hybridization express differently between only expressed liver, but did not tissues lowly cirrhotic In cirrhosis HCC, level gradually decreased hardly until late stage HCC. CONCLUSION: Using arrays is an effective find differential genes. a unique expressing descended along initiation which noteworthy further investigations its significance development
sci-hub.st 参考文章(28)
J M Ladero, J A Agundez, A Rodriguez-Lescure, M Diaz-Rubio, J Benitez, RsaI polymorphism at the cytochrome P4502E1 locus and risk of hepatocellular carcinoma. Gut. ,vol. 39, pp. 330- 333 ,(1996) , 10.1136/GUT.39.2.330
NACS Wong, F Rae, KJ Simpson, GD Murray, DJ Harrison, None, Genetic polymorphisms of cytochrome p4502E1 and susceptibility to alcoholic liver disease and hepatocellular carcinoma in a white population: a study and literature review, including meta-analysis. Journal of Clinical Pathology-molecular Pathology. ,vol. 53, pp. 88- 93 ,(2000) , 10.1136/MP.53.2.88
Joerg F. Schlaak, Catharien M. U. Hilkens, Ana P. Costa-Pereira, Birgit Strobl, Fritz Aberger, Anna-Maria Frischauf, Ian M. Kerr, Cell-type and Donor-specific Transcriptional Responses to Interferon-α USE OF CUSTOMIZED GENE ARRAYS Journal of Biological Chemistry. ,vol. 277, pp. 49428- 49437 ,(2002) , 10.1074/JBC.M205571200
Julian A Peterson, Sandra E Graham, A close family resemblance: the importance of structure in understanding cytochromes P450 Structure. ,vol. 6, pp. 1079- 1085 ,(1998) , 10.1016/S0969-2126(98)00109-9
Ju-Seog Lee, Snorri S. Thorgeirsson, Functional and genomic implications of global gene expression profiles in cell lines from human hepatocellular cancer. Hepatology. ,vol. 35, pp. 1134- 1143 ,(2002) , 10.1053/JHEP.2002.33165
Mikihiro Tsutsumi, Akira Takada, Jian-Song Wang, Genetic polymorphisms of cytochrome P4502E1 related to the development of alcoholic liver disease Gastroenterology. ,vol. 107, pp. 1430- 1435 ,(1994) , 10.1016/0016-5085(94)90546-0
Tanaka, Terada, Misawa, Cytochrome P450 2E1: its clinical and toxicological role. Journal of Clinical Pharmacy and Therapeutics. ,vol. 25, pp. 165- 175 ,(2000) , 10.1046/J.1365-2710.2000.00282.X
Inger Johansson, Erik Eliasson, Carina Norsten, Magnus Ingelman-Sundberg, Hydroxylation of acetone by ethanol- and acetone-inducible cytochrome P-450 in liver microsomes and reconstituted membranes FEBS Letters. ,vol. 196, pp. 59- 64 ,(1986) , 10.1016/0014-5793(86)80214-9
Kin-Man Lai, Jiafu Ji, Sandrine Dudoit, Irene O.L. Ng, Matt van de Rijn, David Botstein, Patrick O. Brown, Xin Chen, Siu Tim Cheung, Samuel So, Sheung Tat Fan, Christopher Barry, John Higgins, Gene expression patterns in human liver cancers. Molecular Biology of the Cell. ,vol. 13, pp. 1929- 1939 ,(2002) , 10.1091/MBC.02-02-0023
Barry H. Rumack, Acetaminophen hepatotoxicity: The first 35 years Clinical Toxicology. ,vol. 40, pp. 3- 20 ,(2002) , 10.1081/CLT-120002882