Characterization of 25 monoclonal antibodies to factor VIII-von Willebrand factor: relationship between ristocetin-induced platelet aggregation and platelet adherence to subendothelium
作者： HV Stel , KS Sakariassen , BJ Scholte , EC Veerman , TH van der Kwast
DOI: 10.1182/BLOOD.V63.6.1408.1408
关键词:
摘要: We have studied the role of factor VIII-von Willebrand (FVIII-vWF) in both platelet adherence to subendothelium and ristocetin-induced aggregation using monoclonal antibodies human FVIII-vWF. Twenty-five were obtained, two which directed VIII moiety FVIII-vWF; one these completely inhibited procoagulant activity (FVIII:C). The remaining 23 von ability latter inhibit arterial was investigated with a perfusion model. According number platelets adhering subendothelium, three groups could be discerned: (A) not affecting adherence; (B) that level as observed when Willebrand's disease plasma tested; (C) aggregation. present group C competed for same or closely related epitope(s) on These results demonstrate domain is FVIII-vWF molecule associated support subendothelium. Based observations, it concluded binding reflects, at least part, physiologic mechanism regulating function primary hemostasis.
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