Origin of Late Lactation Protein from β-Lactoglobulin in the Tammar Wallaby

摘要: Two of the dominant whey proteins tammar wallaby, beta-lactoglobulin (BLG) and late lactation protein (LLP), have been positively identified on gradient polyacrylamide gels by N-terminal sequencing. The origin electrophoretic variation present in these has found to be principally genetic. was examined 97 lactating female wallabies, which 10 were followed throughout determine if any developmental existed. only distinguishable difference results from previously described appearance LLP at beginning phase III (approximately 180 days after birth young). Electrophoretic analysis BLG implies that it exists monomeric, dimeric, tetrameric forms. Very strong linkage disequilibrium, indicative close linkage, between alleles Blg Llp loci. Examination their cDNA sequences suggests represent an ancient gene duplication. Both conserved amino acid residues characteristic lipocalins. imply marsupial mode lactation, hence reproduction, is also very ancient.