Anticancer Activity of Novel Plant Extracts and Compounds from Adenosma bracteosum (Bonati) in Human Lung and Liver Cancer Cells

摘要: Cancer is the second leading cause of death globally, and despite advances in drug development, it still necessary to develop new plant-derived medicines. Compared with using conventional chemical drugs decrease side effects induced by chemotherapy, natural herbal medicines have many advantages. The present study aimed discover potential cytotoxicity ethanol extract its derived fractions (chloroform, ethyl acetate, butanol, aqueous) Adenosma bracteosum Bonati. (A. bracteosum) on human large cell lung carcinoma (NCI-H460) hepatocellular (HepG2). Among these fractions, chloroform showed significant activity inhibition proliferation both cancerous cells because presence bioactive compounds including xanthomicrol, 5,4’-dihydroxy-6,7,8,3’-tetramethoxyflavone, ursolic acid which were clearly revealed nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR, Heteronuclear Multiple Bond Coherence, Single Quantum Coherence Spectroscopy) analyses. According radical scavenging capacity, 5,4’-dihydroxy-6,7,8,3’-tetramethoxyflavone compound (AB2) exhibited highest anticancer NCI-H460 HepG2 IC50 values 4.57 ± 0.32 5.67 0.09 µg/mL respectively, followed lower percent at 13.05 0.55 10.00 0.16 µg/mL, respectively (p < 0.05). Remarkably, AB2 increase production reactive oxygen species accompanied attenuation mitochondrial membrane potential, thus inducing activation caspase-3 liver cancer cells. These results suggest that A. a promising source useful products offers opportunities novel drugs.