Differential 12C-/13C-Isotope Dansylation Labeling and Fast Liquid Chromatography/Mass Spectrometry for Absolute and Relative Quantification of the Metabolome
作者： Kevin Guo , Liang Li
DOI: 10.1021/AC900166A
关键词:
摘要: We report a new quantitative metabolome profiling technique based on differential 12C-/13C-isotope dansylation labeling of metabolites, fast liquid chromatography (LC) separation and electrospray ionization Fourier-transform ion cyclotron resonance mass spectrometry (ESI-FTICR MS) detection. An isotope reagent, 13C-dansyl chloride, can be readily synthesized. This along with 12C-dansyl provides simple robust means metabolites containing primary amine, secondary or phenolic hydroxyl group(s). It is shown that offers 1−3 orders magnitude ESI signal enhancement over the underivatized counterparts. Dansylation alters chromatographic behaviors polar ionic normally not retainable reversed phase (RP) column to an extent they retained separated by RPLC high efficiency. There no isotopic effect labeled 12C-/13C-labeled isoforms me...
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