An investigation of Coxiella burnetii and Coxiella-like bacteria in the brown dog tick (Rhipicephalus sanguineus)

摘要: There are a wide variety of animal reservoirs the zoonotic bacterium Coxiella burnetii (C. burnetii), and ticks may play significant role in natural transmission cycle this pathogen. Recently, domestic dogs have been implicated as C. burnetii. Dogs primary hosts Rhipicephalus sanguineus (R. sanguineus), has previously detected these ticks. The objectives study were to identify record R. collected from Australia, investigate prevalence Subsequent this, bacterial microbiome was investigated. IS1111a transposase element gene targeted using qPCR detect DNA sanguineus. Ion Torrent™ Next-Generation Sequencing platform used sequence 16S rDNA In study, 2,577 morphologically identified recorded Northern Territory, South Australia Western Australia. no positive detection subset 31 by qPCR. Next-generation sequencing universal rRNA revealed that sp. present 53/59 (90%) tick pools. sequences compared GenBank submissions 100% match obtained Philippines. A phylogenetic analysis showed it does not group with pathogenic This be non-pathogenic endosymbiont sanguineus, future investigations could aim assess endosymbionts whether causes cross-reactivity immunologic assays for diagnosis Q fever people.