ERs associate with and regulate the production of caveolin: implications for signaling and cellular actions.

摘要: Recent evidence supports the existence of a plasma membrane ER. In many cells, E2 activates signal transduction and cell proliferation, but steroid inhibits signaling growth in other cells. These effects may be related to interactions ER with signal-modulating proteins membrane. It is also unclear how moves Here, we demonstrate purified vesicles from endothelial membranes colocalization caveolae structural coat protein, caveolin-1. human vascular smooth muscle or MCF-7 (human breast cancer) membranes, coimmunoprecipitation shows that associates caveolin-1 -2. Importantly, rapidly differentially stimulates ER-caveolin association cells -2 protein synthesis promoter/luciferase reporter However, caveolin To determine function for caveolin-ER interaction, expressed This stimulated translocation inhibited E2-induced ERK (MAPK) activation. Both functions required scaffolding domain. Depending upon target cell, ERs associate caveolin, modulates this signaling-inhibitory scaffold protein. explain discordant actions various types. addition, capable facilitating (Molecular Endocrinology 16: 100–115, 2002)