Integrating miRNA and gene expression profiling analysis revealed regulatory networks in gastrointestinal stromal tumors.

摘要: Aim: Currently, little is known about differences in miRNA expression between KIT/PDGFRA mutant and wild-type (WT)-SDH deficient gastrointestinal stromal tumors (GIST). This prompted us to perform an integrated multiple profile of mRNA, constructing original miRNA–mRNA regulatory network WT-SDH GIST patients. Patients & methods: Analyses were carried out on versus GIST. Genome-wide gene-expression analysis performed using Agilent Human microarray Affimetrix array, respectively. Results: Three potential networks (IGF1R → miR-139-5p/miR-455/let-7b, cyclin-dependent kinase 6 (CDK6) miR-139-5p/let-7b CD44 miR-330-3p) identified. Conclusion: The miR-139-5p, 455-5p let-7b signature, particular, may represent important therapeutic target GIST, usually characterized by IGF1R overexpression.