Physico-chemical characterization of asolectin-genistein liposomal system: An approach to analyze its in vitro antioxidant potential and effect in glioma cells viability.
作者： Carla Roberta Lopes de Azambuja , Lurdiane Gomes dos Santos , Marisa Raquel Rodrigues , Renan Ferreira Meneses Rodrigues , Elita Ferreira da Silveira
DOI: 10.1016/J.CHEMPHYSLIP.2015.10.001
关键词:
摘要: In this study, the interaction between soy isoflavone genistein and asolectin liposomes was investigated by monitoring effects of on lipidic hydration, mobility, location order. These properties were analyzed following techniques: horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), low-field (1)H nuclear magnetic resonance (NMR), high-field (31)P NMR, zeta potential, differential scanning calorimetry (DSC) UV-vis spectroscopy. The antioxidant antitumoral activities liposomal system also studied. saturation concentration in ASO corresponded to 484 μM. HATR-FTIR results indicated that influences dynamics phosphate, choline, carbonyl acyl chain methylenes groups. At lipid polar head, NMR showed reduces hydration degree phosphate group, as well its mobility. Genistein ordered interfacial evidenced bandwidth analysis. This ordering effect observed hydrophobic region, HATR-FTIR, DSC turbidity responses. concentration, liposome-loaded inhibits peroxidation induced hydroxyl radical 90.9%. at 100 μM decreased C6 glioma cell viability 57% after 72 h treatment. Results an increase vitro incorporation liposomes. data described work will contribute a better understanding natural-source membrane influence biological activities. Furthermore, genistein-based liposomes, which contain natural-sourced lipids, may be promising drug delivery used therapy.
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