Characterisation of aurone biosynthesis in Antirrhinum majus

摘要: Aurones are bright yellow flavonoids produced in petals of a limited range plant species, including Antirrhinum majus. The biosynthesis aurones is thought to occur by the action aureusidin synthase (AUS), and possibly 7-O-glucosyltransferase (A7GT). temporal spatial occurrence AUS A7GT transcript was examined wild-type A. majus two mutant lines; sulfurea, which has increased aurone production petals, violacea, reduced production. abundance similar all three lines, increasing during flower development coincident with coloration. pattern also being spatially restricted inner epidermis face throat lower petal. A new recessive line (CFR1011) greatly parts petal identified ethylmethanesulfonate mutagenesis homozygous sulfurea line. Transcript for not changed CFR1011 compared line, neither were any point mutations detected coding sequences or A7GT. Thus, violacea do seem control through change predicted biosynthetic genes To examine gene regulation further, putative promoter region isolated other flavonoid promoters. number conserved potential regulatory regions identified, particular consensus site MYB-type transcription factors.