Biochemical evidence on positive effects of rolipram a phosphodiesterase-4 inhibitor in malathion-induced toxic stress in rat blood and brain mitochondria

摘要: Malathion is an organophosphate (OP) pesticide that has been shown to induce oxidative stress in brain through the generation of free radicals and alteration cellular antioxidant defense system indepen- dent its anticholinesterase effects. The aim this study was investigate possible protective role rolipram as a selective phosphodiesterase (PDE) type 4 inhibitor, on toxicity malathion, by measur- ing activities mitochondrial plasma peroxynitrite (ONOO ), glutathione peroxidase (GPx), superoxide dismutase (SOD), Mn-SOD, catalase (CAT), lipid peroxidation (LPO) rats. Effective doses malathion (200 mg/kg/day) lg/kg/day) were administered alone or combi- nation for 7 days intraperitoneal injection. At end experiment, mitochondria animals separated. In cells blood plasma, LPO, ONOO , GPx higher group compared with controls. Rolipram ameliorated all mal- athion-induced changes. Plasma CAT decreased malathion-treated while it increased comparing Co-administration improved both plasma. did not alter total SOD Mn-SOD caused significant elevation mitochondria. conclusion, model we employed, large extent, characterized relationships among malathion-induced neurotoxicity, dysfunction, increase systemic local oxidative/nitro- sative brain, respectively. Intracellular cAMP-elevating agents like rolipram, may be considered beneficial protection recovery toxic damage mito- chondria blood.