Leptin receptor gene in the European sea bass (Dicentrarchus labrax): Cloning, phylogeny, tissue distribution and neuroanatomical organization

摘要: In this study, we report the cloning of three transcripts for leptin receptor in European sea bass, a marine teleost economic interest. The two shortest variants, generated by different splice sites, encode all functional extracellular and intracellular domains but missed transmembrane domain. resulting proteins are therefore potential soluble binding leptin. longest transcript (3605 bp), termed sblepr, includes essential transduction signal. Thus, it is proposed as ortholog human LEPR gene, main responsible signalling. Phylogenetic analysis shows sblepr clustered within group 100% bootstrap replicates. neuroanatomical localization expressing cells has been assessed situ hybridization brains bass both sexes during their first sexual maturation. At histological level, distribution pattern brain no clear differences regarding sex or reproductive season. Transcripts have widespread throughout forebrain midbrain until caudal portion hypothalamus. A high signal detected telencephalon, preoptic area, medial basal hypothalamus pituitary gland. more region, identified longitudinal torus. expression observed suggests that very likely to be involved control food intake, energy reserves reproduction