Interleukin-6 is elevated in synovial fluid of patients with focal cartilage defects and stimulates cartilage matrix production in an in vitro regeneration model.

摘要: This study aimed to determine whether, as in osteoarthritis, increased levels of interleukin-6 (IL-6) are present the synovial fluid patients with symptomatic cartilage defects and whether this IL-6 affects regeneration well degenerated knee. concentrations were determined by ELISA conditioned media chondrocytes regenerating cartilage. Chondrocytes obtained from donors defects, healthy osteoarthritic donors. The effect on metabolism resident knee was studied both inhibition endogenous addition IL-6, a model explants presence fluid, respectively. Readout parameters DNA glycosaminoglycan (GAG) content release. Differences between controls blocked or supplemented samples univariate analysis variance using randomized block design. Synovial contained more than (P = 0.001) did not differ production during higher that defect < 0.001). Adding GAG decreased release 0.05). Inhibition showed trend towards 0.06). Our results support modest anabolic role for matrix production. Targeting multiple cytokines, including may be effective improving repair osteoarthritis.