Folate receptor in malignant effusions of ovarian carcinoma
作者： JAN HOLM , STEEN INGEMANN HANSEN , MIMI HØIER-MADSEN , POUL-ERIK HELKJAER , MICHAEL BZOREK
DOI: 10.1111/J.1699-0463.1995.TB01420.X
关键词:
摘要: Binding of 3H-folate in human ovarian adenocarcinoma tissue was a high-affinity type (K approximately 10(10) M-1) and displayed apparent positive cooperatively. A folate receptor also present ascitic fluid pleural effusion. Radioligand dissociation slow at pH 7.4, but rapid 3.5. The analogues methotrexate particular 5-formyltetrahydrofolate acted as inhibitors binding fluid. Ovarian showed immunostaining with rabbit antibodies against milk folate-binding protein. gel filtration diagram contained two peaks radiolabelled (at 25 100 kDa). kDa peak predominant single band 70 seen on SDS-PAGE immunoblotting malignant effusions. concentration specimens could be determined by an immunochemical method (ELISA) utilizing
sci-hub.se 参考文章(33)
P C Elwood, Molecular cloning and characterization of the human folate-binding protein cDNA from placenta and malignant tissue culture (KB) cells. Journal of Biological Chemistry. ,vol. 264, pp. 14893- 14901 ,(1989) , 10.1016/S0021-9258(18)63786-X
Maria I. Colnaghi, Verna Frasca, Leslie R. Coney, Barton A. Kamen, Antonella Tomassetti, Vincent R. Zurawski, Leon Carayannopoulos, Cloning of a tumor-associated antigen: MOv18 and MOv19 antibodies recognize a folate-binding protein. Cancer Research. ,vol. 51, pp. 6125- 6132 ,(1991)
I. Campbell, W. J. Rettig, J. L. Lewis, P. E. Saigo, P. Garin-Chesa, L. J. Old, Trophoblast and ovarian cancer antigen LK26. Sensitivity and specificity in immunopathology and molecular identification as a folate-binding protein. American Journal of Pathology. ,vol. 142, pp. 557- 567 ,(1993)
E Sadasivan, S P Rothenberg, The complete amino acid sequence of a human folate binding protein from KB cells determined from the cDNA. Journal of Biological Chemistry. ,vol. 264, pp. 5806- 5811 ,(1989) , 10.1016/S0021-9258(18)83621-3
P C Elwood, M A Kane, R M Portillo, J F Kolhouse, The isolation, characterization, and comparison of the membrane-associated and soluble folate-binding proteins from human KB cells. Journal of Biological Chemistry. ,vol. 261, pp. 15416- 15423 ,(1986) , 10.1016/S0021-9258(18)66727-4
Tania A. Jones, William D. Foulkes, Ian G. Campbell, John Trowsdale, Folate-binding Protein Is a Marker for Ovarian Cancer Cancer Research. ,vol. 51, pp. 5329- 5338 ,(1991)
N C Kaarsholm, A M Kolstrup, S E Danielsen, J Holm, S I Hansen, Ligand-induced conformation change in folate-binding protein. Biochemical Journal. ,vol. 292, pp. 921- 925 ,(1993) , 10.1042/BJ2920921
J Holm, S I Hansen, M Høier-Madsen, L Bostad, High-affinity folate binding in human choroid plexus. Characterization of radioligand binding, immunoreactivity, molecular heterogeneity and hydrophobic domain of the binding protein. Biochemical Journal. ,vol. 280, pp. 267- 271 ,(1991) , 10.1042/BJ2800267
R S Verma, A C Antony, Immunoreactive folate-binding proteins from human saliva. Isolation and comparison of two distinct species. Biochemical Journal. ,vol. 286, pp. 707- 715 ,(1992) , 10.1042/BJ2860707
R. Anderson, B. Kamen, K. Rothberg, S. Lacey, Potocytosis: sequestration and transport of small molecules by caveolae Science. ,vol. 255, pp. 410- 411 ,(1992) , 10.1126/SCIENCE.1310359