Galactolipid DGDG and Betaine Lipid DGTS Direct De Novo Synthesized Linolenate into Triacylglycerol in a Stress-Induced Starchless Mutant of Chlamydomonas reinhardtii.

摘要: The increasing demand for triacylglycerol (TAG) enriching polyunsaturated fatty acids (PUFAs) has led to a surge of interest in microalgal TAG metabolism. Polar membrane lipids serve as the desaturation carrier PUFA, and functional group PUFA can be incorporated into TAG. Monogalactoglycerolipid been found provide de novo synthesized oleate acyl or nascent diacylglycerol backbone biosynthesis model green alga, Chlamydomonas reinhardtii. However, whether other take part formation PUFA-attached not clearly discovered. A time course study glycerolipidomics starchless mutant C. reinhardtii, BAFJ5, which hyper-accumulates TAG, revealed that digalactosyldiacylglycerol (DGDG) diacylglycerol-N,N,N-trimethylhomoserine (DGTS) turned main components lipids, accounting 62% total polar under nitrogen deprivation combined with high light conditions. In addition, lipid molecules DGDG 18:3n3/16:0 DGTS 16:0/18:3n6 were presumed involved consecutive integration linolenates Based on stoichiometry calculation, demonstrated major contribution accumulation linolenate-attached Our gives insights potential pathway mediated by turnover Chlamydomonas.