Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay

摘要: The comet assay, also called the single-cell gel electrophoresis (SCGE) is a rapid and sensitive method for detection of DNA damage (strand breaks alkali-labile sites) in individual cells. assay based on embedding cells agarose, their lysis alkaline buffer finally subjection to an electric current. In present study, SCGE was used evaluate extent primary repair peripheral blood lymphocytes workers employed pesticide production. After period high exposure, occupationally exposed manifested increased tail length moment compared control group. spent 6 months out exposure zone, both endpoints were still above that but significantly decreased as results first analysis.