Qualitative and quantitative differences in DNA extracted from different body parts of Apis spp. (Hymenoptera:Apidae) and its validation using microsatellite markers
作者： P D Kamala Jayanthi , V Sridhar , R Vani , P V Rami Reddy , S Sudhagar
DOI:
关键词:
摘要: To study the adaptability and genetic diversity in Apis species, molecular analyses play vital role for which good quality/ quantity of DNA is an important component. Here we describe a simple efficient method isolating quality genomic from different body parts species through modifying previous protocols. Alteration was made extraction buffer (100 mM Tris-HCl, pH 8.0, containing 2% Cetyltrimethylammonium bromide (CTAB), 1.5 M NaCl, 10 Ethylenediaminetetraacetic acid (EDTA) â-mercaptoethanol added after grinding) developed protocol successfully tested on two A. cerana florea. isolated thorax yielded compared to other that further validated using microsatellite markers.
aapmhe.in参考文章(14)
RAPID DETECTION OF THE AFRICANIZED HONEY BEE: A TOOL FOR INDONESIAN ANIMAL QUARANTINE Biotropia: The Southeast Asian Journal of Tropical Biology. ,vol. 16, ,(2009) , 10.11598/BTB.2009.16.1.65
Ana Maria Waldschmidt, Tânia Maria Fernandes Salomão, Everaldo Gonçalves de Barros, Lúcio de Antônio Oliveira Campos, Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponinae) Brazilian Journal of Genetics. ,vol. 20, pp. 421- 423 ,(1997) , 10.1590/S0100-84551997000300011
P de La Rúa, U E Simon, A C Tilde, R F A Moritz, S Fuchs, MtDNA variation in Apis cerana populations from the Philippines Heredity. ,vol. 84, pp. 124- 130 ,(2000) , 10.1046/J.1365-2540.2000.00646.X
Greg J. Hunt, Robert E. Page, Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee. Theoretical and Applied Genetics. ,vol. 85, pp. 15- 20 ,(1992) , 10.1007/BF00223839
Kimberley Christopher, William Clark, An Introduction to DNA: Spectrophotometry, Degradation, and the 'Frankengel' Experiment ,(2001)
Nicolas Châline, Francis L.W. Ratnieks, Nigel E. Raine, Nichola S. Badcock, Terry Burke, Non-lethal sampling of honey bee, Apis mellifera, DNA using wing tips Apidologie. ,vol. 35, pp. 311- 318 ,(2004) , 10.1051/APIDO:2004015
Ting Ji, Guohong Chen, Ling Yin, Genetic diversity and population structure of Chinese honeybees ( Apis cerana ) under microsatellite markers African Journal of Biotechnology. ,vol. 10, pp. 1712- 1720 ,(2011) , 10.5897/AJB10.753
Pamela G. Gregory, Thomas E. Rinderer, Non-destructive sources of DNA used to genotype honey bee (Apis mellifera) queens Entomologia Experimentalis Et Applicata. ,vol. 111, pp. 173- 177 ,(2004) , 10.1111/J.0013-8703.2004.00164.X
P. Sean Walsh, David A. Metzger, Russell Higuchi, Chelex 100 as a Medium for Simple Extraction of DNA for PCR-Based Typing from Forensic Material BioTechniques. ,vol. 10, pp. 506- 513 ,(2013) , 10.2144/000114018
Jayanthi PD, Kamala, R. Rajinikanth, P Sangeetha, KV Ravishankar, A Arthikirubha, Thangam S Devi, Abraham Verghese, Rapid isolation of high molecular weight DNA from single dry preserved adult beetle of Cryptolaemus montrouzieri for polymerase chain reaction (PCR) amplification African Journal of Biotechnology. ,vol. 11, pp. 15654- 15657 ,(2012) , 10.5897/AJB12.1714