Graft-versus-host disease in extremity transplantation: digital image analysis of bone marrow in situ.

摘要: The development of either unstable immune chimerism and lethal graft-versus-host disease or stable alloimmune tolerance can result from extremity transplantation. LBN rats served as recipients Lewis vascularized (limb) transplants. Recipients received no suppression were immunologically unmodified. bone marrow transplanted contralateral limbs was analyzed in situ for distribution nuclei, nuclear area, staining intensity by digital image analysis computerized morphometry. Cellularity significantly increased, fat content decreased the animals' versus tolerant both limbs. Tolerant animals demonstrated increased compared with Additionally, there significant changes between host cellularity within groups. differences disease-positive suggested that autoimmune dysregulation reactions effect donor compartments. Cellular alterations recipients' suggestive subtle subclinical responses.