Comparison and Validation of Putative Pathogenicity-Related Genes Identified by T-DNA Insertional Mutagenesis and Microarray Expression Profiling in Magnaporthe oryzae

摘要: High-throughput technologies of functional genomics such as T-DNA insertional mutagenesis and microarray expression profiling have been employed to identify genes related pathogenicity in Magnaporthe oryzae. However, validation the functions individual identified by these high-throughput approaches is laborious. In this study, we compared two published lists putatively M. oryzae (comprising 1024 genes) 236 genes), respectively, then validated some overlapped between knocking them out using method target gene replacement. Surprisingly, only 13 were lists, none four selected from exhibited visible phenotypic changes on vegetative growth, asexual reproduction, infection ability their knockout mutants. Our results suggest that both might contain large proportions unrelated therefore comparing hardly helpful for identification are more likely be involved initially expected.