Hydrogen peroxide affects specific epithelial subpopulations in cultured rabbit lenses.
作者： B M Wirebaugh , J R Reddan , D C Dziedzic , F J Giblin , J L Peters
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摘要: Purpose. To investigate the effect of hydrogen peroxide on epithelial cells cultured rabbits lenses. Methods. Lenses were in minimum essential medium containing a single dose 0.03, 0.1, or 0.2 mM H 2 O . Three hours later was replaced with peroxide-free medium. also treated 0.5 mlM 1,3-bis(2-chloroethyl)-1 nitrosourea (BCNU) to lower activity glutathione reductase and then exposed 0.03 maintained nearly constant by glucose oxidase. After treatment, lenses fixed whole mounts epithelium prepared processed for electron microscopy. Results. Cells appeared normal; 0.1 not cytotoxic. Exposure elicited swelling pre-equatorial region (30 minutes) followed formation islands at 1 hour. Central normal hour, swollen 3 dead 24 hours. By 48 hours, found central regions. peripheral did exhibit cytotoxicity. If pretreated BCNU challenged level , cytotoxicity induced survived BCNU-H treatment. Conclusions. more resistance than The antioxidant defense repair systems -induced damage do appear be uniformly distributed subpopulations lens
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