Effects Of Polyamines On Vibrio Cholerae Virulence Properties

摘要: Vibrio cholerae is the causative agent of severe enteric disease cholera. To cause cholera bacterium must be able to synthesize both toxin (CT) and toxin-coregulated pilus (TCP) which mediates autoagglutination required for colonization small intestine. Only a few environmental signals have been shown regulate V. virulence gene expression. Polyamines, are ubiquitous in nature, implicated regulating expression other bacteria, not extensively studied their effect on properties. The objective this study was test several polyamines that abundant human intestine All tested inhibited O1 classical strain concentration dependent manner. Putrescine cadaverine decreased synthesis major pilin subunit, TcpA, spermidine increased its production, spermine had no effect. led decrease increase, respectively, relative abundance TCP found cell surface. Spermine reduction whereas none an did affect pili bundling morphology, but caused CTXφ transduction, indicating present surface may fully functional. We hypothesize inhibition likely by positively charged amine groups electrostatically disrupting pili-pili interactions mediate autoagglutination. Our results implicate protective function against cholerae.