RNA interference-mediated knockdown of translationally controlled tumor protein induces apoptosis, and inhibits growth and invasion in glioma cells

摘要: Translationally controlled tumor protein (TCTP) is a highly conserved, growth‑associated and small molecule protein, which expressed in various types of cell. TCTP can promote the growth suppress apoptosis cels. However, few studies have reported effects gliomas. In present study, glioma cell line was established, stably transfected with short hairpin ribonucleic acid (shRNA), to investigate impact downregulated expression on proliferation, invasion cells. Western blot reverse transcription-quantitative polymerase chain reaction analyses demonstrated that shRNA effectively reduced U251 line. MTT colony formation assays revealed significantly inhibited proliferation. Cell cycle analysis using flow cytometry cells pRNA‑H1.1‑TCTP group were arrested G0/G1 phase cycle. detected levels cyclins, including Cyclin D1, E B. Annexin V‑fluorescein isothiocyanate/propidium iodide Hoechst staining apoptotic rate higher than pRNA‑H1.1‑control group, upregulated B-cell-associated X cleaved‑caspase‑3 B-cell lmyphoma-2 process. Wound healing Transwell migration invasiveness cells; activities matrix metalloproteinase (MMP)‑2 MMP‑9 also affected. conclusion, study proliferation invasion, induced These results suggested may be important development metastasis. Therefore, expected become an effective target for gene therapy.