High-throughput screening of catalytically inactive mutants of protein tyrosine phosphatases (PTPs) in a phosphopeptide microarray

作者: Hongyan Sun , Lay Pheng Tan , Liqian Gao , Shao Q. Yao

DOI: 10.1039/B817853D

关键词:

摘要: We report herein a novel phosphopeptide microarray capable of noncovalently “trapping” catalytically inactive mutants protein tyrosine phosphatases (PTPs), and its application in high-throughput determination PTP substrate specificity.

参考文章(18)
Nicholas K. Tonks, Protein tyrosine phosphatases: from genes, to function, to disease Nature Reviews Molecular Cell Biology. ,vol. 7, pp. 833- 846 ,(2006) , 10.1038/NRM2039
Michael C. Sweeney, Anne-Sophie Wavreille, Junguk Park, Jonathan P. Butchar, Susheela Tridandapani, Dehua Pei, Decoding protein-protein interactions through combinatorial chemistry: sequence specificity of SHP-1, SHP-2, and SHIP SH2 domains. Biochemistry. ,vol. 44, pp. 14932- 14947 ,(2005) , 10.1021/BI051408H
Sheng Zhang, Lan Chen, Sanjai Kumar, Li Wu, David S. Lawrence, Zhong-Yin Zhang, An affinity-based fluorescence polarization assay for protein tyrosine phosphatases Methods. ,vol. 42, pp. 261- 267 ,(2007) , 10.1016/J.YMETH.2007.02.008
Sébastien Wälchli, Xavier Espanel, Axel Harrenga, Mario Rossi, Gianni Cesareni, Rob Hooft van Huijsduijnen, Probing Protein-tyrosine Phosphatase Substrate Specificity Using a Phosphotyrosine-containing Phage Library Journal of Biological Chemistry. ,vol. 279, pp. 311- 318 ,(2004) , 10.1074/JBC.M307617200
A. J. Flint, T. Tiganis, D. Barford, N. K. Tonks, Development of “substrate-trapping” mutants to identify physiological substrates of protein tyrosine phosphatases Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 1680- 1685 ,(1997) , 10.1073/PNAS.94.5.1680
Yan-Li Song, Megan L. Peach, Peter P. Roller, Su Qiu, Shaomeng Wang, Ya-Qiu Long, Discovery of a novel nonphosphorylated pentapeptide motif displaying high affinity for Grb2-SH2 domain by the utilization of 3'-substituted tyrosine derivatives. Journal of Medicinal Chemistry. ,vol. 49, pp. 1585- 1596 ,(2006) , 10.1021/JM050910X
Mahesh Uttamchandani, Wei L Lee, Jun Wang, Shao Q Yao, None, Quantitative inhibitor fingerprinting of metalloproteases using small molecule microarrays. Journal of the American Chemical Society. ,vol. 129, pp. 13110- 13117 ,(2007) , 10.1021/JA073914V
Maja Köhn, Marta Gutierrez-Rodriguez, Pascal Jonkheijm, Stefan Wetzel, Ron Wacker, Hendrik Schroeder, Heino Prinz, Christof M. Niemeyer, Rolf Breinbauer, Stefan E. Szedlacsek, Herbert Waldmann, A microarray strategy for mapping the substrate specificity of protein tyrosine phosphatase. Angewandte Chemie. ,vol. 46, pp. 7700- 7703 ,(2007) , 10.1002/ANIE.200701601
Mathieu Garaud, Dehua Pei, Substrate profiling of protein tyrosine phosphatase PTP1B by screening a combinatorial peptide library. Journal of the American Chemical Society. ,vol. 129, pp. 5366- 5367 ,(2007) , 10.1021/JA071275I