Resident enteric flora modulates the development of colitis in gnotobiotic mice

摘要: Background: Previous investigations from this laboratory have shown that infection of C3H strain mice with Brachyspira hyodysenteriae induces a typhlocolitis features are similar to ulcerative colitis. The immunological response these conventional C3H/HeOuJ has been be predominant Thl-like (e.g., antigen-specific IgG2a responses and IFN-y responses). While the spirochetal pathogen initiates onset disease, model bacterial-induced colitis dependent upon nature resident bacterial flora host's inflammatory response. Aim: To characterize immune defined gastrointestinal in pathogenesis experimental Methods: Gnotobiotic C3H/HeN possessing an altered schaedler (8 separate organisms) were used evaluate following challenge B. hyodysenteriae. Immunologic parameters evaluated included cecal morphology, histologic lesion severity scores, isotype serum antibody (ELISA), antigen-induced cytokine responses. Results: Results indicate disease is slower less gnotobiotic comparison complex, flora. immunologic subsequent was mixed Thl :Th2 phenotype, but predominantly Th2-like. Because colony colonized Helicobacter species, we co-infected H. bilis assess influence Helicobacters on bias colonization did enhance colonic lesions Conclusions: These data significantly