A novel cell-containing device for regenerative medicine: biodegradable nonwoven filters with peripheral blood cells promote wound healing

摘要: The efficacy of skin regeneration devices consisting nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human enhanced their production growth factors, such as transforming factor β1 (TGF-β1) vascular endothelial factor, when they were captured on filters. Cells biodegradable expected to serve a local supply factors cell sources placed in wounded skin. Nonwoven made polylactic acid (PLA) cut out 13-mm disks into cell-capturing devices. Mouse filtered, resulting PLA with mouse peripheral (m-PBCs) at capture rates 65.8 ± 5.2 %. Then, the attached full-thickness surgical wounds diabetic db/db mouse 14 days model severe chronic wounds. area treated m-PBCs (PLA/B+) reduced 8.5 ± 12.2 % compared day 0, although non-treated control showed reduction only 60.6 ± 27.8 %. However, without increased 162.9 ± 118.7 %. By histopathological study, PLA/B+ groups more effectively accelerated formation epithelium. keratinocyte (FGF-7) TGF-β1 productions vitro, which may be related This device is useful adaptable another application.