Zur funktion der ciliaten-riesenchromosomen: Nachweis von RNS-polymerase in den polytänen chromosomen der makronukleusanlage von Stylonychia mytilus

摘要: Summary In freeze-dried squash-preparations of Stylonychia mytilus macronuclei from vegetative animals as well macronuclear Anlagen young exkonjugants synthetize RNA when incubated in a medium containing 3 H-UTP and the other three nucleosid-triphosphates. Micronuclei do not show any synthesis. contrast to RNA-Polymerase-activity enzyme activity giant-chromosome stage can be inhibited completely by 5 μ g/ml α -Amanitin so is identified RNA-polymerase II. First detectable 20 – 25 hours old excon-jugants more intensive this than older ones which have large polytene chromosomes. It discussed that technique used investigation probably allows conclusion living exconjugants contain active RNA-polymerase. So giant chromosomes only function preparing genome but additionally should busy transcription.