Terminal adenylation in the synthesis of RNA by Q beta replicase.
作者： J N Bausch , F R Kramer , E A Miele , C Dobkin , D R Mills
DOI: 10.1016/S0021-9258(18)33084-9
关键词:
摘要: We investigated the apparent requirement that Q beta replicase must add a nontemplated adenosine to 3' end of newly synthesized RNA strands. used abbreviated MDV-1 (+)-RNA templates lacked either 62 or 63 nucleotides at their 5' in reactions. The (-)-RNA strands from these (+)-strand were released replication complex, yet they did not possess 3'-terminal adenosine. These results imply that, despite observations all naturally occurring RNAs by 3'-adenosine, addition an extra is obligate step for release completed Since normal end, it probable particular sequence template required terminal adenylation occur.
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