Cytochemical characterization of microvillar and perimicrovillar membranes in the posterior midgut epithelium of Rhodnius prolixus.

摘要: Perimicrovillar membranes (PMM) are structures present on the surface of midgut epithelial cells hematophagous insect, Rhodnius prolixus. They cover microvilli and especially evident 10 days after blood meal, providing compartmentalization enzymatic processes in intestinal microenvironment. Using an enzyme cytochemical approach, Mg2+-ATPase ouabain-sensitive Na+K+-ATPase activities were observed plasma (or microvillar) membrane (MM) PMM. In contrast, alkaline phosphatase was only detected MM. cationized ferritin colloidal iron hydroxide particles, anionic sites found luminal fluorescein isothiocyanate (FITC)-labeled lectins, residues alpha-d-galactose, mannose, N-acetyl-neuraminic acid, N-acetyl-d-galactosamine N-acetyl-galactosamine-alpha-1,3-galactose apical posterior cells. On other hand, using FITC-labeled neoglycoproteins (NGP) it possible to detect presence carbohydrate binding molecules (CBM) recognizing N-acetyl-d-galactosamine, alpha-d-mannose, alpha-l-fucose alpha-d-glucose epithelium. The use digitonin showed sterols MM These results have led authors suggest that for some components PMM resembles lining R. prolixus, composing a system which covers stretches space.