Two simple programs for the analysis of data from enzyme-linked immunosorbent (ELISA) assays on a programmable desk-top calculator.

摘要: Abstract We have designed two programs for use with an inexpensive programmable calculator which rapidly and accurately convert raw data generated from enzyme-linked immunosorbent assays directly into antigen concentration. The first program computes compares effective doses (ED50)'s between a standard each unknown sample assayed. ED50 the is then multiplied by concentration factor yields second linearizes sigmoidal assay titration curve using logit-log transformation of in order to compute values. Both employ stringent limit conditions decrease “nonsense” calculations. Data are processed least-squares best-fit linear regression analysis.