Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein

摘要: Chromatin structure in transcribed regions poses a barrier for intragenic transcription. In comprehensive study of the yeast chromatin remodelers and Mot1p-NC2 regulators TATA-binding protein (TBP), we detected synthetic genetic interactions indicative suppression Conditional depletion Mot1p or NC2 absence ISW1 remodeler, but not other remodelers, activated cryptic FLO8 promoter. Likewise, conditional deletion backgrounds H3K36 methyltransferase Set2p Asf1p-Rtt106p histone H3-H4 chaperones, important factors involved maintaining repressive environment, resulted increased transcripts. Activity promoter is associated with reduced H3 levels, TBP binding tri-methylation H3K4 independent Spt-Ada-Gcn5-acetyltransferase function. These data reveal cooperation negative regulation specific to inhibit