An Experimental Study on the Mechanical and Biological Properties of Bio-Printed Alginate/Halloysite Nanotube/Methylcellulose/Russian Olive-Based Scaffolds.

摘要: Purpose: Cartilage shows neither repairs nor regenerative properties after trauma or gradual wear and causes severe pain due to bones rubbing. Bioprinting of tissue-engineered artificial cartilage is one the most fast-growing sciences in this area that can help millions people against disease. Methods: proper bioscaffolds for repair was main goal study. The bioprinting process achieved by a novel composition consisting alginate (AL), Halloysite nanotube (HNT), methylcellulose (MC) prepared bio-ink. Also, effect Russian olive (RO) chondrocytes growth on also investigated work. Compressive, hardness viscosity tests, Energy-Dispersive X-Ray Spectroscopy (EDX), Fourier-Transform Infrared (FT-IR), Differential Scanning Calorimetry (DSC), water-soluble Tetrazolium (WST) assay, transmission electron microscopy (TEM) scanning (SEM) were carried out. Results: results show constant concentrations AL, MC, RO (20 mg/ml 20 10 RO) when concentration HNT increased from (T-7) (T-8) compressive stiffness 241±45 kPa 500.66±19.50 kPa. AL saved water content chondrocyte produced good higher printing resolution. Conclusion: living cell efficiency 11% bioprinted scaffolds comparison with control group without RO. Results obtained through in-vivo studies similar those in-vitro studies. According results, T-7 bio-ink has potential repairs.