Short-term study of allogeneic retinal pigment epithelium transplants onto debrided Bruch's membrane.

摘要: PURPOSE. To investigate the survival and behavior of retinal pigment epithelium (RPE) microaggregates transplanted onto hydraulically debrided Bruch's membrane to compare results using three different vehicles for cell delivery. METHODS. RPE obtained from male cats were tapetal area female after native was debrided. For control, one introduced into debridements. Each transplant or control specimen analyzed histologically immunohistochemically. Transplanted cells identified by in situ labeling cat Y chromosome. RESULTS. Histologically, significant numbers condensed, darkly stained nuclei observed all transplants compared with few TUNEL-positive cells. Cellular retinal-dehyde-binding protein present up day 7 transplants. In both specimens, antibody against Ki-67 nuclear antigen labeled some at 3. outer layer most frequently 1, but much less frequent days specimens. CONCLUSIONS. appeared retain least markers differentiation surgery. Some proliferation also seen. Apoptotic death RPE, as judged TUNEL staining rarely. imposed no adverse effect on overlying retina. be similar each