The Role of the Wnt/PCP Formin Daam1 in Renal Ciliogenesis
作者： Mark E. Corkins , Vanja Krneta-Stankic , Malgorzata Kloc , Pierre D. McCrea , Andrew B. Gladden
DOI: 10.1101/512533
关键词:
摘要: Kidneys are composed of numerous ciliated epithelial tubules called nephrons. Each nephron functions to reabsorb nutrients and concentrate waste products into urine. Defects in primary cilia associated with abnormal formation nephrons cyst a wide range kidney disorders. Previous work Xenopus laevis zebrafish embryos established that loss components make up the Wnt/PCP pathway, Daam1 ArhGEF19 (wGEF) perturb tubulogenesis. Dishevelled, which activates both canonical non-canonical affect multiciliated cells. In this study, we investigated role noncanoncial renal ciliogenesis utilizing polarized mammalian epithelia cells (MDCKII IMCD3) embryonic kidney. We demonstrate knockdown MDCKII IMCD3 leads cilia, Daam1s effect on is mediated by formin-activity Daam1. Moreover, co-localizes ciliary transport protein IFT88. Interestingly, knocking down does not lead cilia. This data suggests new for
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