Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression.

摘要: Abstract Sequences that comprise the 244-base-pair polyomavirus enhancer region are also required in cis for viral DNA replication (Tyndall et al., Nucleic Acids Res. 9:6231-6250, 1981). We have studied relationship between sequences activate and those enhance transcription two ways. One approach, recently described by de Villiers al. (Nature [London], 312:242-246, 1984), which was replaced with other or cellular transcriptional enhancers suggested an function is replication. The this paper, to analyze a series of deletion mutants functionally dissect enabled us localize four sequence elements involved activation These elements, little homology, redundant. Element A (nucleotides 5108 through 5130) synthesized as 26-mer XhoI sticky ends, one more copies were introduced into plasmid containing origin replication, but lacking region. Whereas copy activated only 2 5% wild-type level, inserted either orientation completely restored found multiple active measuring beta-globin mRNA levels expressed from contained site 3' gene. observed correlation number 26-mers level RNA expression.