Experimental and theoretical studies of the optimisation of fluorescence from near-infrared dye-doped silica nanoparticles

摘要: There is substantial interest in the development of near-infrared dye-doped nanoparticles (NPs) for a range applications including immunocytochemistry, immunosorbent assays, flow cytometry, and DNA/protein microarray analysis. The main motivation this work significant increase NP fluorescence that may be obtained compared with single dye label, example Cy5. Dye-doped NPs were synthesised reduction as function concentration was correlated occurrence homo-Forster resonance energy transfer (HFRET) NP. Using standard analytical expressions describing HFRET, we modelled loading. results confirmed HFRET which arises from small Stokes shift dyes provided simple method predicting optimum loading maximum fluorescence. We used inverse micelle to prepare monodispersed silica NPs. characterised using dynamic light scattering, UV spectroscopy, transmission electron microscopy (TEM). quantum efficiency inside NPs, loading, also determined. fluorescent measured approximately 165 times brighter than free dye, at an optimal 2% (w/w). These experimental good agreement model predictions.