The effects of phenylmethylsulfonyl fluoride on inositol-acylation and fatty acid remodeling in African trypanosomes.

摘要: Phenylmethylsulfonyl fluoride (PMSF) has been shown to inhibit the addition of ethanolamine phosphate glycosylphosphatidylinositol (GPI) intermediates in Trypanosoma brucei (Masterson, W. J., and Ferguson, M. A. J. (1991) EMBO 10, 2041-2045). Here we show that Man3-GlcN-PI intermediate accumulates presence PMSF can undergo fatty acid remodeling, suggesting remodeling enzymes are not specific for phosphate-containing GPI intermediates. We also inhibits acylation inositol residue bloodstream form T. brucei. Pulse-chase experiments demonstrate glycolipid C (ethanolamine-PO4-Man3-GlcN-(acyl)PI) is an obligatory precursor A (ethanolamine-PO4-Man3-GlcN-PI) be converted These data suggest a model where terminal product biosynthetic pathway, dynamic equilibrium with The inhibition by was observed procyclic forms but mammalian HeLa cells. results differences between relevant parasite enzymes.