Development of Hydra lacking nerve and interstitial cells

摘要: Hydra attenuata were rendered free of interstitial cells (I cells) and cell derivatives by colchicine treatment. These hydra then cloned cultivated for 18 months their developmental capacities studied. Some experimental possessed a few (about 1% the normal numbers) retained this low level during prolonged culture active growth without differentiation I-cells into specialized cells. Other completely freed Maceration histological analyses showed that once is all it does not recover them, nor do its buds contain I cell-free also lack nerve cells, nematocytes, gametes endodermal gland tissue consists only ectodermal epithelial lacking grow, bud, exhibit renewal patterns, regenerate preserve polarity generally typical hydra. hypostomal has inductive capacity, as tissue, when implanted in or gastric tissue. Regenerating undergoes precocious determination Only some quantitative aspects develop abnormally. We conclude consisting are capable essentially development.