A new method to induce myasthenia gravis models and the protective effect of soluble decay accelerating factors.

摘要: It is expensive to induce experimental autoimmune myasthenia gravis (EAMG) by active immunity, and difficult obtain natural acetylcholine receptor (AChR). We sought a new method of inducing EAMG immunizing rats with artificially synthesized AChR. The AChR mRNA in TE671 cells was extracted reverse transcribed. inclusion body purified protein concentration determined, the animal model used for induction. serum from rat blood. antibody titer determined using enzyme-linked immunosorbant assay (ELISA). decay accelerating factor (DAF) ELISA, metabolism rDAF western blot. evaluated inhibition determining 50% complement hemolysis unit serum. extracellular domain (ECD) nucleotide sequence clone produced polymerase chain reaction completely consistent that human gene bank; it induced isopropyl β-D-1-thiogalactopyranoside express after insertion into vector pET16b. Sodium dodecyl sulfate polyacrylamide gel electrophoresis demonstrated exact target. ECD able bind mAb35 dialysis renaturation, which activity. soluble immunize models. inhibitory effect unsatisfactory owing high rate injection easy obtaining AChRTEα1 subunit substitution method.