Association of neuropeptide Y Y1 receptors with glutamate‐positive and NPY‐positive neurons in rat hippocampal cultures

摘要: The hippocampus is particularly enriched with neuropeptide tyrosine (NPY) and NPY receptors including the Y1, Y2 Y5 subtypes. We have previously reported on enrichment of cultured rat hippocampal neurons in specific [125I][Leu31, Pro34]PYY/BIBP3226-sensitive (Y1) binding sites Y1 receptor mRNAs [St-Pierre et al. (1998) Br. J. Pharmacol., 123, p183]. now identified which cell types express receptor. majority receptors, visualized using either radiolabeled probe [125I][Leu31,Pro34]PYY or two antibodies directed against distinct domains receptor, was expressed as revealed by neuron-specific enolase (NSE) immunostaining. One antibody second extracelllular loop (amino acids 185-203) whereas intracellular C-terminal 355-382). labelling evident over both perikarya processes. Neurons labelled various probes were mostly glutamate-positive double Most interestingly, a number NPY-positive also suggesting that this subtype may act an autoreceptor to regulate release hippocampus. These results thus provide anatomical basis for modulation glutamate