Prevalence and patterns of Y chromosome microdeletion in infertile men with azoospermia and oligzoospermia in Northeast China

摘要: Background: In some cases infertile men showed small deletions of specific genes in the Y chromosome. It had been confirmed, these deleted are greatly associated with spermatogenic failure. However, frequency and patterns such microdeletions among not clearly clarified. Objective: We sought to determine chromosome azoospermic oligozoospermic Northeast China, try optimize selection sequence tagged sites (STSs) AZF multiplex polymerase chain reaction (PCR). Materials Methods: 720 330 men, from China were included this retrospective study during May 2008 November 2012. Semen analysis was performed according World Health Organization guidelines. detected by assays. G-banding method used for Karyotype analysis. Chi-square tests compare patients. Results: Of 1050 12.95% shown microdeletions, 19.43% abnormal chromosomal karyotype. Deletions AZFc region most frequent 75.00%, followed AZFb 13.33%, AZFbc 09.62%, AZFa 2.22%. All patients presence sY84, sY86, sY127, sY134. Deletion sY127 (p=0.0101) sY157 (p=0.0043) significant difference between group group. Conclusion: may relate azoospermia while can be ignored screening patients.