Studies on cytoplasmic ribonucleic acid from rat liver I. Fractionation and function of soluble ribonucleic acid
作者： L. Bosch , H. Bloemendal , M. Sluyser
DOI: 10.1016/0006-3002(60)90041-X
关键词:
摘要: Abstract 1. The soluble polynucleotides isolated by phenol extraction from rat-liver cytoplasm were fractionated on the resin Ecteola. 2. Four fractions obtained, one of which (s3-RNA) could be transferred to microsomal RNA in presence enzymes, ATP, GTP and a nucleoside triphosphate regenerating system. efficiency this transfer was about 17% based isotope content added polynucleotides. 3. On incubation pH 5 enzymes with DL -[14C]leucine or mixture radioactive amino acids s3-RNA became mainly labelled. Minor amounts radioactivity, not exceeding 10% that recovered s3-RNA, also detected other fractions. 4. further characterized ultracentrifugal analysis, dialysis treatment EDTA. 5. possible significance these results for mechanism protein biosynthesis microsomes is discussed.
elsevier.com
sci-hub.se 参考文章(36)
Mary G. Hamilton, Mary L. Petermann, Ultracentrifugal studies on ribonucleoprotein from rat liver microsomes. Journal of Biological Chemistry. ,vol. 234, pp. 1441- 1446 ,(1959) , 10.1016/S0021-9258(18)70027-6
John W. Littlefield, Elizabeth B. Keller, INCORPORATION OF C14-AMINO ACIDS INTO RIBONUCLEOPROTEIN PARTICLES FROM THE EHRLICH MOUSE ASCITES TUMOR Journal of Biological Chemistry. ,vol. 224, pp. 13- 30 ,(1957) , 10.1016/S0021-9258(18)65006-9
John W. Littlefield, Elizabeth B. Keller, Jerome Gross, Paul C. Zamecnik, Studies on cytoplasmic ribonucleoprotein particles from the liver of the rat. Journal of Biological Chemistry. ,vol. 217, pp. 111- 124 ,(1955) , 10.1016/S0021-9258(19)57162-9
H. Bloemendal, Starch Electrophoresis Journal of Chromatography A. ,vol. 2, pp. 121- 135 ,(1959) , 10.1016/S0021-9673(01)86272-5
G. E. W. Wolstenholme, Cecilia M. O'Connor, Ciba Foundation Symposium on the Regulation of Cell Metabolism ,(2011)
Mahlon B. Hoagland, Mary Louise Stephenson, Jesse F. Scott, Liselotte I. Hecht, Paul C. Zamecnik, A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN SYNTHESIS Journal of Biological Chemistry. ,vol. 231, pp. 241- 257 ,(1958) , 10.1016/S0021-9258(19)77302-5
Paul C. Zamecnik, Elizabeth B. Keller, Relation between phosphate energy donors and incorporation of labeled amino acids into proteins. Journal of Biological Chemistry. ,vol. 209, pp. 337- 354 ,(1954) , 10.1016/S0021-9258(18)65561-9
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
T. Hultin, Alexandra Von Der Decken, The transfer of soluble polynucleotides to the ribonucleic acid of rat liver microsomes. Experimental Cell Research. ,vol. 16, pp. 444- 447 ,(1959) , 10.1016/0014-4827(59)90279-4
L. Bosch, H. Bloemendal, M. Sluyser, Metabolic inter-relationships between soluble and microsomal RNA in ratliver cytoplasm. Biochimica et Biophysica Acta. ,vol. 34, pp. 272- 274 ,(1959) , 10.1016/0006-3002(59)90267-7