Permethylated N-glycan analysis with mass spectrometry.
作者： Zhenxin Lin , David M. Lubman
DOI: 10.1007/978-1-62703-392-3_12
关键词:
摘要: Protein glycosylation plays an important role in multiple cell functions, and aberrations of protein are associated with various malignancies including cancer. In this chapter, we provide a detailed protocol for MALDI MS analysis permethylated N-glycans extracted from human serum proteins. The includes procedures N-glycan purification in-solution permethylation, structural elucidation by MALDI-QIT-TOF MS, construction indices to quantify levels certain types glycosylation, such as fucosylation, which may serve potential disease biomarker.
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