Identification of T and B Cell Subpopulations in Human Peripheral Blood: Electrophoretic Mobility Distributions Associated with Surface Marker Characteristics

摘要: The electrophoretic mobility distributions of human peripheral blood lymphocytes isolated on Ficoll-Hypaque gradients were characterized by laser Doppler spectroscopy. Three major subpopulations spectrally resolved, due to differences in their mobility, when an electric field was applied the scattering cuvette. fastest component (centered at 2.35 µm/sec/V/cm for 25°C, 0.28 M sucrose medium 0.005 ionic strength) passed through nylon fiber columns and identified as a T cell subpopulation. slowest (1.85 µm/sec/V/cm) which further enriched one-step rosette procedure with sheep erythrocytes, reacted activated-complement (C3) antiserum immunoglobulins therefore B intermediate 2.15 appears be another Although cells this did not pass columns, they erythrocytes. Furthermore, these appear have surface or complement receptors.