Differentiation of two human neuroblastoma cell lines alters SV2 expression patterns
作者： Helgi B. Schiöth , Helgi B. Schiöth , Robert Fredriksson , Emilia Lekholm , Mikaela M. Ceder
DOI: 10.1186/S11658-020-00243-8
关键词:
摘要: The synaptic vesicle glycoprotein 2 (SV2) family is essential to the machinery involved in neurotransmission and recycling. isoforms SV2A, SV2B SV2C are implicated neurological diseases such as epilepsy, Alzheimer’s Parkinson’s disease. Suitable cell systems for studying regulation of these proteins essential. Here we present gene expression data two human neuroblastoma lines after differentiation. Human SiMa IMR-32 were treated seven days with growth supplements (B-27 N-2), all-trans-retinoic acid (ATRA) or vasoactive intestinal peptide (VIP) levels SV2 neuronal targets analyzed. reacted differently treatments, only one three was affected at a time. choline O-acetyltransferase (CHAT) changed concert supplement treatment, decreasing cells while increasing IMR-32. ATRA treatment resulted no detected changes either line VIP increased both dopamine transporter (DAT) cells. synergistic patterns between CHAT well DAT mirror connectivity found disease models knock-out animals, although here genetic alteration made. These differentiation treatments could possibly be used study function.
biomedcentral.com
springer.com
scilit.net参考文章(63)
Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, Sandra Ceccatelli, Neural Stem Cells for Developmental Neurotoxicity Studies Methods of Molecular Biology. ,vol. 758, pp. 67- 80 ,(2011) , 10.1007/978-1-61779-170-3_5
Doris M. Benbrook, Pierre Chambon, Cécile Rochette-Egly, Mary Ann Asson-Batres, History of Retinoic Acid Receptors Subcellular Biochemistry. ,vol. 70, pp. 1- 20 ,(2014) , 10.1007/978-94-017-9050-5_1
SM Bajjalieh, GD Frantz, JM Weimann, SK McConnell, RH Scheller, Differential expression of synaptic vesicle protein 2 (SV2) isoforms The Journal of Neuroscience. ,vol. 14, pp. 5223- 5235 ,(1994) , 10.1523/JNEUROSCI.14-09-05223.1994
Karen Niederreither, Vemparala Subbarayan, Pascal Dollé, Pierre Chambon, Embryonic retinoic acid synthesis is essential for early mouse post-implantation development. Nature Genetics. ,vol. 21, pp. 444- 448 ,(1999) , 10.1038/7788
Jo Vandesompele, Katleen De Preter, Filip Pattyn, Bruce Poppe, Nadine Van Roy, Anne De Paepe, Frank Speleman, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Genome Biology. ,vol. 3, pp. 1- 12 ,(2002) , 10.1186/GB-2002-3-7-RESEARCH0034
Markus Höltje, Agnieszka Münster-Wandowski, Gudrun Ahnert-Hilger, Synaptic vesicle proteins: targets and routes for botulinum neurotoxins. Current Topics in Microbiology and Immunology. ,vol. 364, pp. 159- 177 ,(2012) , 10.1007/978-3-642-33570-9_8
James A. Waschek, Stephen A. Wank, Anthony J. Harmar, Illana Gozes, Laurent Journot, Marc Laburthe, Akira Arimura, Patrick Robberecht, Joseph R. Pisegna, Stephen R. Rawlings, Sunil P. Sreedharan, Sami I. Said, International Union of Pharmacology. XVIII. Nomenclature of Receptors for Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide Pharmacological Reviews. ,vol. 50, pp. 265- 270 ,(1998)
Zhenze Zhao, Xiuye Ma, Tzu-Hung Hsiao, Gregory Lin, Adam Kosti, Xiaojie Yu, Uthra Suresh, Yidong Chen, Gail E Tomlinson, Alexander Pertsemlidis, Liqin Du, None, A high-content morphological screen identifies novel microRNAs that regulate neuroblastoma cell differentiation Oncotarget. ,vol. 5, pp. 2499- 2512 ,(2014) , 10.18632/ONCOTARGET.1703
Louise Dickson, Keith Finlayson, VPAC and PAC receptors: From ligands to function. Pharmacology & Therapeutics. ,vol. 121, pp. 294- 316 ,(2009) , 10.1016/J.PHARMTHERA.2008.11.006
Fatema J. Serajee, Ahm M. Huq, Homozygous Mutation in Synaptic Vesicle Glycoprotein 2A Gene Results in Intractable Epilepsy, Involuntary Movements, Microcephaly, and Developmental and Growth Retardation. Pediatric Neurology. ,vol. 52, pp. 642- 646 ,(2015) , 10.1016/J.PEDIATRNEUROL.2015.02.011