Development of a Novel Electrochemical Biosensor Based on Carbon Nanofibers-Gold Nanoparticles-Tyrosinase for the Detection of Ferulic Acid in Cosmetics.

摘要: The present paper deals with the electrochemical behavior of three types sensors based on modified screen-printed electrodes (SPEs): a sensor carbon nanofibers (CNF/SPE), gold nanoparticles (CNF-GNP/SPE) and biosensor tyrosinase (CNF-GNP-Ty/SPE). To prepare biosensor, (Ty) was immobilized surface electrode already nanoparticles, by drop-and-dry technique. properties were studied cyclic voltammetry in electroactive solutions, position shape active redox peaks are according to nature materials modifying electrodes. In case ferulic acid, series characteristic observed, processes being more intense for higher sensitivity selectivity due immobilization tyrosinase, specific enzyme phenolic compounds. calibration curve subsequently created using CNF-GNP-Ty/SPE acid solutions various concentrations range 0.1–129.6 μM. This new allowed low values detection threshold quantification limit, 2.89 × 10−9 mol·L−1 9.64 mol·L−1, respectively, which shows that electroanalytical method is feasible quantifying real samples. quantitatively determined cosmetic products means biosensor. results obtained validated spectrometric infrared range, differences between two methods under 5%.