Altered inhibitory innervation of circular smooth muscle in Crohn's colitis

摘要: To determine whether decreased tissue vasoactive intestinal polypeptide levels might affect inhibitory neural input, fresh colonic specimens were obtained from patients with Crohn's colitis (n = 7) and normal subjects 13). Immunoreactive measured in the muscularis externa by radioimmunoassay localized sections immunostaining. Circular muscle strips maintained an organ bath; junction potentials evoked short- long-duration field stimulation resting membrane recorded using intracellular impalements. In colitis, displayed a bimodal distribution which 3 had greater than or equal to 4 SE lower mean specimens. levels, immunoreactive material was absent circular layer myenteric plexus. Mean potentials, amplitude of short-duration stimulation, initial not different between two groups. However, 60th potential during prolonged (p less 0.01) (6 mV) compared (11 mV). These results show that diminished input associated extractable staining nerve fibers containing polypeptide.