Fluorescence lifetime imaging in scanning microscopes: Acquisition speed, photon economy and lifetime resolution
作者： H. C. Gerritsen , M. A. H. Asselbergs , A. V. Agronskaia , W. G. J. H. M. Van Sark
DOI: 10.1046/J.1365-2818.2002.01031.X
关键词:
摘要: In this paper a detailed discussion is presented of the factors that affect fluorescence lifetime imaging performance scanning microscope equipped with single photon counting based, two- to eight-channel, time-gated detection system. particular we discuss sensitivity, resolution, acquisition speed, and shortest lifetimes can be measured. Detection systems four eight time-gates are significantly more sensitive than two time-gate Only minor sensitivity differences were found between or time-gates. Experiments confirm resolution dominated by statistics. The time response detector determines resolved; about 25 ps for fast MCP-PMTs 300-400 other detectors. maximum count rate MCP-PMTs, however, 10-100 times lower PMTs. Therefore, speed MCP-PMT based limited. With PMT operated close its able record image beating myocyte in less one second.
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