Effects on fertility of motile sperm to egg ratio with use of cryopreserved Rhamdia quelen semen at different post-activation times.
作者： Giovano Neumann , Paulo Vanderlei Sanches , Robie Allan Bombardelli
DOI: 10.1016/J.ANIREPROSCI.2019.01.001
关键词:
摘要: Abstract The aim in the present study was to evaluate effects of motile sperm:oocyte ratio and use thawed spermatozoa at different post-activation times artificial reproduction gray catfish (Rhamdia quelen). Cryopreserved sperm samples were evaluated for motility velocity using Computer Assisted Sperm Analysis (CASA). activation curves generated a non-linear statistical model used assess after thawing. Thus, oocytes mixed with 70,000, 90,000, 110,000, 130,000, 150,000 170,000. 11, 16 30 s activation. At these times, velocities corresponded 52, 37 21 μm/s. experimental factors (spermatozoa:oocyte time activation) on oocyte fertilization, egg hatching, percentage normal larvae evaluated. response surface analysis indicated there no interaction (P > 0.05) between spermatozoa:oocyte hatching or larvae. activation, however, affected (P
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