Nuclear localization of Semliki Forest virus-specific nonstructural protein nsP2.

摘要: Abstract About 50% of Semliki Forest virus-specific nonstructural protein nsP2 is associated with the nuclear fraction in virus-infected BHK cells. Transport into nucleus must be specific, since only trace amounts nsP3 and nsP4 about 13% nsP1, all derived from same polyprotein, were found nucleus. Subfractionation [35S]methionine-labeled cells showed that 80 to 90% was matrix. Indirect immunofluorescence, anti-nsP2 antiserum, most intensive staining structures which by Nomarski optics appeared nucleoli. In presence 1 5 micrograms dactinomycin per ml nuclei stained evenly no nucleoli could found. occurred early infection fairly rapid. A cDNA encoding complete isolated polymerase chain reaction technique ligated a simian virus 40 expression vector derivative. When transfected this pSV-NS2 lipofection procedure, expressed 5% cells, as shown indirect immunofluorescence. positively immunofluorescence stain Thus, have information directs it matrix and, more specifically,