CitA (citrate) and DcuS (C4-dicarboxylate) sensor kinases in thermophilic Geobacillus kaustophilus and Geobacillus thermodenitrificans.

作者: Sabrina Graf , Constanze Broll , Juliane Wissig , Alexander Strecker , Maria Parowatkin

DOI: 10.1099/MIC.0.000171

关键词:

摘要: The thermophilic Geobacillus thermodenitrificans and kaustophilus are able to use citrate or C4-dicarboxylates like fumarate succinate as the substrates for growth. genomes of sequenced strains (nine strains) each encoded a two-component system CitA family. sensor kinase G. (termed CitAGt) was replace Escherichia coli (CitAEc) in heterologous complementation assay restoring expression CitAEc-dependent citC-lacZ reporter gene anaerobic growth on citrate. Complementation specific DcuSGk) DcuSEc E. coli. It responded citrate, suggesting that DcuSGk is, DcuSEc, C4-dicarboxylate with side-activity DcuSGk, unlike homologous DctS from Bacillus subtilis, required no binding protein function assay. Thus, contain kinases DcuS type, respectively, retain substrate specificity under mesophilic conditions

参考文章(49)
Kei Asai, Sang-Hoon Baik, Yasuhiro Kasahara, Shigeki Moriya, Naotake Ogasawara, Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis. Microbiology. ,vol. 146, pp. 263- 271 ,(2000) , 10.1099/00221287-146-2-263
José María Landete, Luisa García-Haro, Amalia Blasco, Paloma Manzanares, Carmen Berbegal, Vicente Monedero, Manuel Zúñiga, Requirement of the Lactobacillus casei MaeKR two-component system for L-malic acid utilization via a malic enzyme pathway. Applied and Environmental Microbiology. ,vol. 76, pp. 84- 95 ,(2010) , 10.1128/AEM.02145-09
Fabian Sievers, Andreas Wilm, David Dineen, Toby J Gibson, Kevin Karplus, Weizhong Li, Rodrigo Lopez, Hamish McWilliam, Michael Remmert, Johannes Söding, Julie D Thompson, Desmond G Higgins, Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega Molecular Systems Biology. ,vol. 7, pp. 539- 539 ,(2011) , 10.1038/MSB.2011.75
Michele Magrane, UniProt Consortium, None, UniProt Knowledgebase: a hub of integrated protein data Database. ,vol. 2011, ,(2011) , 10.1093/DATABASE/BAR009
Tamara N. Nazina, Elena V. Lebedeva, Andrei B. Poltaraus, Tatyana P. Tourova, Alexandre A. Grigoryan, Diyana Sh. Sokolova, Anatoliy M. Lysenko, George A. Osipov, Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. International Journal of Systematic and Evolutionary Microbiology. ,vol. 54, pp. 2019- 2024 ,(2004) , 10.1099/IJS.0.02932-0