作者: Sabrina Graf , Constanze Broll , Juliane Wissig , Alexander Strecker , Maria Parowatkin
DOI: 10.1099/MIC.0.000171
关键词:
摘要: The thermophilic Geobacillus thermodenitrificans and kaustophilus are able to use citrate or C4-dicarboxylates like fumarate succinate as the substrates for growth. genomes of sequenced strains (nine strains) each encoded a two-component system CitA family. sensor kinase G. (termed CitAGt) was replace Escherichia coli (CitAEc) in heterologous complementation assay restoring expression CitAEc-dependent citC-lacZ reporter gene anaerobic growth on citrate. Complementation specific DcuSGk) DcuSEc E. coli. It responded citrate, suggesting that DcuSGk is, DcuSEc, C4-dicarboxylate with side-activity DcuSGk, unlike homologous DctS from Bacillus subtilis, required no binding protein function assay. Thus, contain kinases DcuS type, respectively, retain substrate specificity under mesophilic conditions